Stable isotope analysis measures the ratios of specific chemical isotopes in bone collagen.

The most commonly used isotopes are carbon (comparing the ratios of carbon-12 and carbon-13) and nitrogen (comparing the ratios of nitrogen-14 and nitrogen-15).

These ratios reflect the composition of the diet — specific isotope ratios are characteristic of specific types of food, and the isotope ratios in bone collagen reflect the average diet over the years during which that collagen was being formed.

The carbon isotope ratios in Mesolithic hunter-gatherers from Britain typically indicate a diet based primarily on terrestrial mammals, with some contribution from freshwater resources in some individuals.