When?

Were there multiple mixing events?

What happened to the purely Ancient North Eurasian population — did it persist into the post-glacial period, or was it absorbed entirely into later populations?

How many founding populations contributed to the ancestry of the Americas, and what were their relative sizes and compositions?

These questions have been the drivers of research in the decade since the 2014 publication.

Each new ancient genome from Siberia, Beringia, or the Americas adds evidence that constrains the possible answers.