For a child living on the Seward Peninsula in the early Holocene, the expected isotopic signature would reflect a diet based on C3 plant resources and on the animals that consumed those plants — consistent with the Arctic and subarctic ecosystems of the region.

Nitrogen stable isotopes in teeth reflect the position of an individual in the food chain.

Higher nitrogen isotope ratios indicate consumption of protein from higher trophic levels — animals that consume other animals, rather than plants directly.

For a very young child, the nitrogen isotope signal is complicated by the specific physiological context: breastfeeding mothers and their infants share a specific isotopic relationship, and the nitrogen signature of a nursing infant reflects both the diet of the mother and the physiological process of breastfeeding itself.

Whether stable isotope analysis was conducted on the Trail Creek tooth as part of the published research program is not certain from the available documentation.