These refinements do not undermine the core conclusion — that the Clovis child was ancestral to modern Native Americans — but they add nuance to the understanding of specifically which modern populations are most closely related to the Clovis founding population.

The relationship between the Anzick genome and the genomes of ancient South Americans has been particularly informative.

As discussed in the context of the Population Y debate and the Lagoa Santa site, some South American populations show genetic signals that require explanation beyond a simple model of Clovis-derived ancestry.

The Anzick genome provides an important reference point for these analyses, as the genome of a confirmed Clovis individual helps calibrate expectations for what Clovis-derived ancestry should look like in descendant populations.

Isotopic analysis of the Anzick remains — the study of the ratio of different stable isotopes of elements like strontium, oxygen, and carbon, which can reveal information about diet and geographic origin — has also been applied to the Anzick material.