The 64 Maya Boys Buried Beneath Chichén Itzá — And The DNA Test That Found Two Pairs Of Identical Twins
The 100 percent success rate for the 64 sampled individuals in the 2024 study reflects both the specific preservation environment of the chultún and the targeting of the petrous bone for sampling.
The specific decision to sample the left petrous bone — rather than either petrous bone or the most accessible one — was motivated by the need to avoid duplicate sampling.
In a commingled burial where individual remains are not neatly separated, the safest way to ensure that each sample represents a different individual is to apply a rule that any one person can supply only one sample.
Since everyone has exactly one left petrous bone, this rule guarantees uniqueness among the samples analyzed.